Effects of nitrogen addition and seasonal change on arbuscular mycorrhizal fungi community diversity in a poplar plantation

Arbuscular mycorrhizal (AM) fungi play a crucial role in carbon (C), nitrogen (N), and phosphorous (P) biogeochemical cycling. Therefore, it is essential to determine the seasonal responses of AM fungal community N addition understanding better ecological processes against background intensified deposition. Based on an ongoing field simulation experiment with five levels (0, 5, 10, 15, 30 gN·m −2 ·a −1 ) 5-year-old poplar plantation at Dongtai Forest Farm Yancheng, Jiangsu province, eastern China, soil physicochemical properties, root colonization rate, rhizosphere diversity composition four seasons (summer, autumn, winter, spring) were investigated. Meanwhile, relationships between characteristics environmental factors analyzed. High-throughput sequencing showed that dominant genera Glomus (average relative abundance 87.52%), Diversispora (9.62%), Acaulospora (1.85%). The significantly increased rate spring. (Chao Shannon indexes) was primarily affected by change rather than addition, summer lower other three seasons. Redundancy analysis temperature, available P, total pH structure community. It can be concluded influenced whereas had notable effect diversity. Although greatly composition, exerted more substantial addition. These results will improve our underground ecosystems.